Description
Intended Uses: This anti PM-Scl ELISA is a solid phase enzyme immunoassay employing recombinant human 100 kDa PM-Scl for the quantitative and qualitative detection of antibodies against PM-Scl in human serum.
Principle of the Assay: Samples diluted 1:101 are incubated in the microplates coated with the specific antigen. The antibodies, if present in the specimen, bind to the antigen. The unbound fraction is washed off in the following step. Afterwards anti-human immunoglobulins conjugated to horseradish peroxidase (conjugate) are incubated and react with the antigen-antibody complex of the samples in the microplates. Unbound conjugate is washed off in the following step. Addition of TMBsubstrate generates an enzymatic colorimetric (blue) reaction, which is stopped by diluted acid (color changes to yellow). The intensity of color formation from the chromogen is a function of the amount of conjugate bound to the antigen-antibody complex and this is proportional to the initial concentration of the respective antibodies in the sample.
Background: The polymysositis/scleroderma i. e. PM/Scl antigen was first described by Reichlin and Mathioli in 1976. PM/Scl antibodies can be found in sera from individuals suffering from polymyositis/ scleroderma overlap syndrome (PM/Scl). The two major PM-Scl antigens are proteins of 100kDa and 75kDa, termed PM-Scl 100 and PM-Scl 75, respectively. Most anti-PM/Scl-positive sera are reactive with the 100 kDa protein, while some also recognize the 75 kDa protein. The PM-Scl 75 and PM-Scl 100 antigens are part of a multiprotein particle also termed PM-Scl (formerly PM-1). The cellular function of the complete PM-Scl particle has long been an enigma. Recently, the human PM/Scl complex was shown to be related to the yeast exosome, a complex which functions in very diverse pathways, all dealing with RNA degradation, such as mRNA turnover, rRNA processing and sn(o)RNA biogenesis